Title:
STEM CELL AGGREGATE SUSPENSION COMPOSITION AND DIFFERENTIATION METHOD
Document Type and Number:
Japanese Patent JP2018148933
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method for producing a cell aggregate derived from primate pluripotent stem cells suspended in a physiologically acceptable medium.SOLUTION: The method for producing cells for transplantation comprises the following steps: (a) a step of seeding an hES single cell suspension in a bioreactor; a step of forming an hES cell aggregate by agitating the hES single cell suspension; and (c) a step of forming a bioreactor that contains an hES-derived cell aggregate that has differentiated in suspension due to contact of the hES cell aggregate with a differentiation agent.SELECTED DRAWING: Figure 23
Inventors:
CHAD GREEN
YU XIAOJIE
BANG ANNE
BRANDON EUGENE P
OLIVIA KELLY
ALAN AGULNICK
EDWARD BAETGE
KEVIN D'AMOUR
SCHULTS THOMAS C
ROBINS ALLAN J
YU XIAOJIE
BANG ANNE
BRANDON EUGENE P
OLIVIA KELLY
ALAN AGULNICK
EDWARD BAETGE
KEVIN D'AMOUR
SCHULTS THOMAS C
ROBINS ALLAN J
Application Number:
JP2018128583A
Publication Date:
September 27, 2018
Filing Date:
July 05, 2018
Export Citation:
Assignee:
VIACYTE INC
International Classes:
C12N5/0735; C12N5/071
Domestic Patent References:
| JP2012507281A | 2012-03-29 |
Foreign References:
| WO2005123902A1 | 2005-12-29 |
Attorney, Agent or Firm:
Yoshiyuki Kawaguchi
Sakai Shigeru
Sakai Shigeru