Title:
PRODUCTION METHOD OF cDNA
Document Type and Number:
Japanese Patent JP2020150937
Kind Code:
A
Abstract:
To provide methods for producing cDNA from RNA contained in a plant sample by a simple operation without using expensive reagents.SOLUTION: Provided is a production method of cDNA, the method comprising: (1) a crushing step of crushing a plant sample in a crushing solution containing a reducing agent at a concentration of 10 mM or more; and (2) a reverse transcription step of performing a reverse transcription reaction in a solution that contains the crushed product and a reverse transcriptase and has a volume of 3 μL or more, and the purification of nucleic acid from the plant sample or the crushed product being not performed.SELECTED DRAWING: None
Inventors:
KASHIMA MAKOTO
NAGANO ATSUSHI
NAGANO ATSUSHI
Application Number:
JP2020024537A
Publication Date:
September 24, 2020
Filing Date:
February 17, 2020
Export Citation:
Assignee:
UNIV RYUKOKU
International Classes:
C12P19/34
Attorney, Agent or Firm:
Atomi International Patent Office