Title:
【発明の名称】インターリューキン-2およびその純化方法
Document Type and Number:
Japanese Patent JP2704512
Kind Code:
B2
Abstract:
Interleukin 2 (IL 2; T-cell growth factor), produced with and without costimulation by Burkitt's lymphoma line Daudi, is purified approximately 37,000-fold to apparent homogeneity from lymphocyte-conditioned medium by (NH4)2SO4- precipitation, ion-exchange chromatography, gel filtration and hydrophobic chromatography. Native IL 2 produced in the absence of Daudi cells has a molecular weight of about 26,000 daltons as measured by gel filtration and yields IL 2 having two molecular weights of about 16,000 and 17,000 daltons after denaturation as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. IL 2 produced in the presence of Daudi cells shows a molecular weight of approximately 14,500 daltons as measured by both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Inventors:
Roland Meltersmann
Carle Berthe
Salvato-Le Benuta
Carle Berthe
Salvato-Le Benuta
Application Number:
JP6988083A
Publication Date:
January 26, 1998
Filing Date:
April 20, 1983
Export Citation:
Assignee:
Slown-Kettering In-Staying-Foot-Cancer-Reseach
International Classes:
A61K38/00; A61P35/00; A61P37/00; C07K1/20; C07K14/435; C12P21/00; C07K14/47; C07K14/52; C07K14/54; C07K14/55; C12R1/91; (IPC1-7): C12P21/00; C07K14/47
Other References:
【文献】J.Exp.Med,Vol.152,P.1709 (1980.12)
Attorney, Agent or Firm:
Morio Sada