Title:
【発明の名称】抗腫瘍活性物質
Document Type and Number:
Japanese Patent JP2721854
Kind Code:
B2
Abstract:
PURPOSE: To obtain the new antitumor active substance GIF having a specific amino acid sequence, having an action for specifically inhibiting the multiplication of tumor cells against the tumor cells, and useful as an antitumor agent, etc., by culturing immune-charging cells originated from a mammalian to induce and produce the substance. CONSTITUTION: An antitumor activity substance GIF contains an amino acid sequence of the formula as its constituting protein, exhibits a single protein absorbance peak coincident with the activity of' the GIF in a negative phase high performance liquid chromatography, is migrated as a single band at the position of a mol.wt. of 17-18K in a SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in the presence of 2-mercaptoethanol, exhibits an isoelectric point (PI) of approximately 6.5-7.02 in an isoelectric point electrophoresis (IEF) and has a tumor cell multiplication-inhibiting activity. The GIF is obtained by culturing human peripheral blood lymphocytes in a medium containing an antitumor substance-inducing agent, kidney bean lectin, at 37 deg.C for 3 days in a carbon dioxide culture device, separating the product from the supernatant of the culture solution as a raw solution material, and subsequently purifying the product.
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Inventors:
NAKAI SATORU
KANEDA MAYUMI
KIKUMOTO YOSHIKAZU
KO HIDEMITSU
KAWAI KAZUYOSHI
TAKEKATA SETSUKO
ISHII KYOSHI
YANAGIHARA YASUO
HIRAI YOSHIKATSU
KANEDA MAYUMI
KIKUMOTO YOSHIKAZU
KO HIDEMITSU
KAWAI KAZUYOSHI
TAKEKATA SETSUKO
ISHII KYOSHI
YANAGIHARA YASUO
HIRAI YOSHIKATSU
Application Number:
JP35332595A
Publication Date:
March 04, 1998
Filing Date:
December 29, 1995
Export Citation:
Assignee:
OOTSUKA SEIYAKU KK
International Classes:
C12N15/09; A61K38/00; A61P35/00; C07K1/20; C07K14/435; C12P21/02; C12R1/19; (IPC1-7): C07K14/435; C12N15/09; C12P21/02
Domestic Patent References:
| JP5993093A |
Other References:
ＮＡＴＵＲＥ ＶＯＬ．３１５，２０ ＪＵＮＥ １９８５，Ｐ．６４１−６４７
Attorney, Agent or Firm:
Eiji Saegusa (2 others)