Title:
単純放射状免疫拡散アッセイのデジタル画像
Document Type and Number:
Japanese Patent JP4575919
Kind Code:
B2
Abstract:
A method for performing a single radial immunodiffusion (SRID) assay for an antigen or antibody of interest, comprising the steps of: (a) applying one or more test sample(s) to a gel, wherein the gel contains (i) antibody specific to the antigen of interest and/or (ii) antigen recognised by the antibody of interest; (b) allowing the sample(s) to diffuse radially from its/their point(s) of application; (c) preparing a digital image of the gel after diffusion has taken place; (d) determining the external diameter(s) of precipitation halos within the digital image; (e) comparing the determined diameters to those resulting from one or more standard sample(s) containing a known concentration of the antigen or antibody of interest; and, optionally, (f) using the results from step (e) to determine the concentration(s) of the antigen or antibody of interest in the test sample(s).
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Inventors:
France, France
Application Number:
JP2006530761A
Publication Date:
November 04, 2010
Filing Date:
October 04, 2004
Export Citation:
Assignee:
Chiron Sochieta A Responsor Billita Limitata
International Classes:
G01N33/559; G01N33/543; G01N33/558; G01N33/569; G01N33/68
Domestic Patent References:
| JP2003522802A | ||||
| JP3191848A | ||||
| JP4072547A |
Foreign References:
| DE4318692A1 |
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita