Title:
耐熱性L-リボースイソメラーゼとその製造方法並びに用途
Document Type and Number:
Japanese Patent JP5103597
Kind Code:
B2
Abstract:
Object: To provide a thermostable L-ribose isomerase. Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45°C, optimal pH of pH 9. 0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45°C during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.
Inventors:
Ken Izumori
Kenji Morimoto
Goro Takada
Tokuda Masaaki
Yoshio Tsujisaka
Kei Takeshita
Keiji Tsuzaki
Kazuhiro Okuma
Kenji Morimoto
Goro Takada
Tokuda Masaaki
Yoshio Tsujisaka
Kei Takeshita
Keiji Tsuzaki
Kazuhiro Okuma
Application Number:
JP2008545316A
Publication Date:
December 19, 2012
Filing Date:
March 26, 2007
Export Citation:
Assignee:
National University Corporation Kagawa University
Rare sugar production technology research institute
Hayashibara Co., Ltd.
Matsutani Chemical Industry Co., Ltd.
Rare sugar production technology research institute
Hayashibara Co., Ltd.
Matsutani Chemical Industry Co., Ltd.
International Classes:
C12N9/90; C12P19/02; C07H3/02; C12N1/20
Other References:
Int. J. Syst. Evol. Microbiol., vol. 51, pages 925-932 (2001)
Antimicrob. Agents Chemother., vol. 48, pages 305-312 (2004)
Biotechnol. Bioeng., vol. 22, pages 1957-1977 (1980)
Biochim. Biophys. Acta, vol. 1521, pages 141-145 (2001)
Antimicrob. Agents Chemother., vol. 48, pages 305-312 (2004)
Biotechnol. Bioeng., vol. 22, pages 1957-1977 (1980)
Biochim. Biophys. Acta, vol. 1521, pages 141-145 (2001)
Attorney, Agent or Firm:
Asako Sudo
Akinobu Sudo
Akinobu Sudo
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