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Patent Searching and Data


Title:
タンパク質を含有する試料の分析方法
Document Type and Number:
Japanese Patent JP6741259
Kind Code:
B2
Abstract:
Provided is a method for analyzing a protein-containing sample. The method comprises (1) dissolving a probe capable of non-specifically interacting with a plurality of proteins in a plurality of solvents having different ionic strengths and/or pH levels; (2) adding a protein-containing sample to a plurality of probe solutions prepared in the step (1), thereby the proteins in the sample and the probe are interacted non-specifically; (3) measuring the fluorescence intensities of the plurality of probe solutions to which the protein-containing sample was added in the step (2); and (4) comparing the pattern of fluorescence intensities obtained in the step (3) with the pattern of fluorescence intensities obtained from a reference sample.

Inventors:
Shunsuke Tomita
Ishihara Saayaka
Ryoji Kurita
Application Number:
JP2018550276A
Publication Date:
August 19, 2020
Filing Date:
November 10, 2017
Export Citation:
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Assignee:
National Institute of Advanced Industrial Science and Technology
International Classes:
G01N21/64; C09K11/06; G01N33/48; G01N33/68
Domestic Patent References:
JP9183853A
Other References:
TOMITA, Shunsuke, et al.,The use of an enzyme-based sensor array to fingerprint proteomic signatures of sera from different mammalian species,Analytical Sciences,2016年 2月10日,Volume 32, Issue 2,Pages 237-240,URL,https://doi.org/10.2116/analsci.32.237
TOMITA, Shunsuke et al.,Artificial modification of an enzyme for construction of cross-reactive polyion complexes to fingerprint signatures of proteins and mammalian cells,Analytical chemistry,2016年 8月22日,Volume 88,Pages 9079-9086,URL,https://doi.org/10.1021/acs.analchem.6b02010
Attorney, Agent or Firm:
Shoichi Okuyama
Matsushima Tetsuo
Ayako Nakamura
Akiko Mizushima
Arihara Koichi