Title:
コレステロールエステラーゼ活性が向上したポリペプチド
Document Type and Number:
Japanese Patent JP7436981
Kind Code:
B2
Abstract:
To provide novel polypeptides.SOLUTION: Disclosed is a polypeptide having 80% or more identity to one of the amino acid sequences represented by SEQ ID NOs: 1-4, where an amino acid corresponding to leucine at position 287 (in the case of amino acid sequences of SEQ ID NOs: 1-3) or at position 286 (in the case of the amino acid sequence of SEQ ID NO:4) is substituted by isoleucine or alanine, and the polypeptide exhibits improved cholesterol esterase activity as compared with the activity before substitution.SELECTED DRAWING: None
Inventors:
Keitaro Yoshida
Yoshiaki Yasutake
Tomohiro Tamura
Shuji Muramatsu
Kenji Konishi
Shinichi Sasegawa
Yoshiaki Yasutake
Tomohiro Tamura
Shuji Muramatsu
Kenji Konishi
Shinichi Sasegawa
Application Number:
JP2020027721A
Publication Date:
February 22, 2024
Filing Date:
February 21, 2020
Export Citation:
Assignee:
National Institute of Advanced Industrial Science and Technology
Asahi Kasei Pharma Corporation
Asahi Kasei Pharma Corporation
International Classes:
C12Q1/44; C12N9/18
Domestic Patent References:
| JP8256767A | ||||
| JP2019205402A | ||||
| JP2011505866A |
Foreign References:
| WO2004048605A1 |
Other References:
Takeda, Y. et al.,"Purification, characterization, and molecular cloning of organic-solvent-tolerant cholesterol esterase from cyclohexane-tolerant Burkholderia cepacia strain ST-200",Extremophiles,2006年,Vol. 10,pp. 269-277
Tomic, S. et al.,"Stereoselectivity of Burkholderia cepacia lipase towards secondary alcohols: molecular modelling and 3D QSAR approach",Tetrahedron: Asymmetry,2004年,Vol. 15,pp. 1163-1172
Tomic, S. et al.,"Stereoselectivity of Burkholderia cepacia lipase towards secondary alcohols: molecular modelling and 3D QSAR approach",Tetrahedron: Asymmetry,2004年,Vol. 15,pp. 1163-1172
Attorney, Agent or Firm:
Yoshiyuki Inaba
Toshifumi Onuki
Akihiko Eguchi
Kazuhiko Naito
Toshifumi Onuki
Akihiko Eguchi
Kazuhiko Naito