Title:
【発明の名称】ポリメラーゼチェイン反応の特異性と簡便性の改良
Document Type and Number:
Japanese Patent JPH05506143
Kind Code:
A
Abstract:
Improvements to the polymerase chain reaction (PCR), a process for in vitro enzymatic amplification of specific nucleic acid sequences, can be achieved by changing the way that PCR reagents are mixed and the enzymatic reaction is started and by the replacement of mineral oil, commonly used as a vapor barrier to minimize solvent evaporation, by a grease or wax. The use of such mixtures allows for the delay of reagent mixing until the first heating step of a PCR amplification, thereby reducing the enzymatic generation of nonspecific products which occurs when a complete mixture of PCR reagents, with or without test sample, stands at room temperature or below. These mixtures increase the shelf-life of PCR reagents and increase protection of the laboratory environment against contamination by PCR product.
Inventors:
Block, will
Raymond, Jonathan
Aki Otsuki
Raymond, Jonathan
Aki Otsuki
Application Number:
JP50457191A
Publication Date:
September 16, 1993
Filing Date:
February 15, 1991
Export Citation:
Assignee:
F. Hoffman-La Roche Age
International Classes:
B01L3/14; B29C59/10; C08J7/046; C12M1/00; C12N15/09; C12Q1/68; G01N35/00; (IPC1-7): C12M1/00; C08J7/04; C12N15/10; C12Q1/68
Attorney, Agent or Firm:
Takashi Ishida (3 others)