Title:
【発明の名称】胎児細胞回収法
Document Type and Number:
Japanese Patent JPH05506144
Kind Code:
A
Abstract:
PCT No. PCT/AU91/00115 Sec. 371 Date Nov. 2, 1992 Sec. 102(e) Date Nov. 2, 1992 PCT Filed Mar. 27, 1991 PCT Pub. No. WO91/14768 PCT Pub. Date Oct. 3, 1991.The present invention provides a method for selectively recovering fetal cells from a maternal blood sample. The method is performed on a blood sample from a pregnant woman having different first and second cell surface antigens expressed by a first allele of a polymorphic genetic locus and a second allele of a polymorphic genetic locus. The method separates maternal and fetal cells based on differential reactivities of the cells to antibodies specific for polymorphic cell surface antigens, particularly the HLA antigens. In particular, the fetal and maternal cells are separated based on the non-reactivity of the fetal cells to an antibody specific for a cell surface antigen encoded by a non-transmitted maternal allele. The method can be performed using solid phase-affixed antibody and recovering non-bound cells or using fluorescent labeled antibody and recovering unlabeled cells by fluorescence-activated cell sorting. In a preferred embodiment, the cells are also contacted with a second antibody specific for the second cell surface antigen. Fetal cells are separated based on their reaction with, at most, one of the antibodies.
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Inventors:
Simons, malcolm the ray
Application Number:
JP50562591A
Publication Date:
September 16, 1993
Filing Date:
March 27, 1991
Export Citation:
Assignee:
Genetype Actien Gezershaft
International Classes:
C12N5/07; C12N5/00; C12N5/073; C12N15/09; G01N33/53; G01N33/569; G01N33/80; C12R1/91; (IPC1-7): C12N5/08; G01N33/53
Attorney, Agent or Firm:
Heikichi Odashima