Title:
【発明の名称】農薬を測定するためのキットおよび方法
Document Type and Number:
Japanese Patent JPH06505880
Kind Code:
A
Abstract:
A test kit and a rapid and sensitive method are provided for the multiple detection of organophosphate and carbamate pesticides. The test method employs an insect brain material that hydrolyzes a 6-substituted D luciferin ester, preferably a 6-substituted acetyl D luciferin ester, to produce D luciferin. Hydrolysis is inhibited by the pesticides in a test sample. Luciferase and adenosine triphosphate react with the D luciferin to produce oxyluciferin and emitted bioluminescence. The brain material is initially incubated with a test sample followed by the addition of the ester, luciferase and adenosine triphosphate. The kit contains the brain material, the ester and optionally luciferase and adenosine triphosphate, and may also contain means for incubating, and means for measuring emitted bioluminescence, and a control standard.
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Inventors:
Somer, Elisa
Saul, Stephen
Charm, stanley yee
Saul, Stephen
Charm, stanley yee
Application Number:
JP51269693A
Publication Date:
July 07, 1994
Filing Date:
January 07, 1993
Export Citation:
Assignee:
Charm Sciences, Inc.
International Classes:
C07D277/64; C12Q1/66; G01N21/76; C07D277/68; G01N33/567; G01N33/60; (IPC1-7): C12Q1/66; C07D277/68; G01N21/76
Attorney, Agent or Firm:
Kyozo Yuasa (6 people outside)