Title:
【発明の名称】遺伝子ファミリーのメンバーのヌクレオチドシーケンスを比較して遺伝子型を決定する方法とそのためのキット
Document Type and Number:
Japanese Patent JPH06511380
Kind Code:
A
Abstract:
PCT No. PCT/US91/07308 Sec. 371 Date Apr. 14, 1993 Sec. 102(e) Date Apr. 14, 1993 PCT Filed Oct. 8, 1991 PCT Pub. No. WO92/08117 PCT Pub. Date May 14, 1993A method is provided for determining a genotype by comparing the nucleotide sequence of members of a gene system which flank the polymorphous sections of a locus or loci of interest. In a general embodiment of the method, the nucleotide sequences chosen for comparison (i) contain one or two sequences that are completely conserved between the members of the gene family, where two or more members are selected from different sources, and (ii) the completely conserved sequences flank strongly conserved sections of genetic material. The completely conserved sequences are typically used to amplify, from different sources, the strongly conserved sections of genetic material. The resulting amplified nucleic acid sequences from the different sources are then compared to establish genotypes.
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Inventors:
Tyranus, Marcel, Gee, Jay
Application Number:
JP51828391A
Publication Date:
December 22, 1994
Filing Date:
October 08, 1991
Export Citation:
Assignee:
The Perkin-Elmer Corporation
International Classes:
C12N15/09; C12Q1/68; C12N15/00; C12Q1/6827; C12Q1/6858; C12Q1/6881; C12Q1/6888; G01N33/50; (IPC1-7): C12Q1/68; C12N15/00
Attorney, Agent or Firm:
Hidesaku Yamamoto