Title:
ANALYTIC METHOD AND ANALYZER OF HUMAN IgG4 ANTIBODY
Document Type and Number:
Japanese Patent JP2022055933
Kind Code:
A
Abstract:
To provide an analytic method of an affinity variant to an Fc receptor in which a result can be obtained with good reproducibility and highly accurately for a human IgG4 antibody whose analysis has conventionally been difficult.SOLUTION: A human IgG4 antibody is analyzed by an analytic method including: a process of passing buffer solution from pH5 to pH7 into a column filled with insoluble carrier in which Fc receptors are immobilized to bring the column into equilibrium; a process of injecting solution containing antibodies into the column which has been brought into equilibrium; and a gradient eluting process of gradually increasing the ratio of buffer solution from pH3 to pH5 while passing the buffer solution from pH5 to pH7 to elute the antibody, in which the buffer solution from pH3 to pH5 has higher buffer agent concentration than the buffer solution from pH5 to pH7.SELECTED DRAWING: Figure 1
Inventors:
TANAKA TORU
Application Number:
JP2020163658A
Publication Date:
April 08, 2022
Filing Date:
September 29, 2020
Export Citation:
Assignee:
TOSOH CORP
International Classes:
C07K1/22; C07K14/735; C07K16/00; C07K16/46; C07K17/00; G01N30/26; G01N30/34; G01N30/54; G01N30/88