Title:
組換え第VIII因子を高発現させるためのBHK細胞
Document Type and Number:
Japanese Patent JP5117542
Kind Code:
B2
Abstract:
The present invention relates to a method for increased production of a secreted, recombinant protein product through the introduction of molecular chaperones in a mammalian host cell. the present invention also relates to a mammalian host cell with enhanced expression of a secreted recombinant protein product by co-expressing at least one chaperone protein.
Inventors:
Chan, Siam-Yoon
Tan, Shin Yi Bet
Tao, Ewen
Woo, Yongjan
Kelly, Ruth
Tan, Shin Yi Bet
Tao, Ewen
Woo, Yongjan
Kelly, Ruth
Application Number:
JP2010143013A
Publication Date:
January 16, 2013
Filing Date:
June 23, 2010
Export Citation:
Assignee:
Nipponex Incorporated
International Classes:
C12N5/10; C07K14/755; C07K14/81; C12N5/00; C12N15/09; C12N15/85; C07K14/47
Domestic Patent References:
| JP2003501014A |
Foreign References:
| WO1994008012A1 |
Other References:
Steven W. Pipe et al.,Differential Interaction of Coagulation Factor VIII and Factor V with Protein Chaperones Calnexin and Calreticulin,Journal of Biological Chemistry,1998年,Vol. 273, No. 14,p. 8537-8544
Attorney, Agent or Firm:
Hironobu Onda
Makoto Onda
Atsushi Honda
Makoto Onda
Atsushi Honda