糖タンパク質を作製するための細胞培養方法

Japanese Patent JP7265494

B2

The instant application provides a method for screening batches of soy hydrolysate for a desired amount of a component thereof, such as ornithine or putrescine, and selecting only those batches of soy hydrolysate that have a desired amount of such component. The present disclosure also sets forth methods for culturing cells in media supplemented with selected batches of soy to produce more consistent, high quality lots of a protein of interest. Further, the present disclosure provides a plurality of protein preparations that have each been produced by culturing cells in media supplemented with separate batches of soy hydrolysate containing a desired amount of ornithine or putrescine, whereby each batch of protein produced exhibits improved quality of the protein of interest or amount of quality protein produced.

JPS62270598	NOVEL TUMOR RELATED ANTIGEN
WO/2000/058492	FRACTIONATION OF SOYBEAN 7S GLOBULIN AND 11S GLOBULIN AND PROCESS FOR PRODUCING THE SAME
WO/1989/005345	GENETIC MODIFICATION OF ENDOTHELIAL CELLS

John Chen
Sean Lawrence
Amy Johnson
Theodore Ronnie
Ravindra Pangur
Tha Chun Han
Scott Carver
Bernhard Schilling
Kathleen Linehan Harrison
Kenneth Wayne Wood
Aaron Wesley Miller

JP2019572719A

April 26, 2023

July 03, 2018

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REGENERON PHARMACEUTICALS, INC.

C12P21/00; C07K19/00; C12N1/00; C12Q1/02

JP2017501749A
JP2017517518A
JP2008517610A

Aoki Atsushi
Shinji Mihashi
Youichi Watanabe
Nakajima Masaru
Ryotaro Takei
Hidenao Tsuda

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