To provide a method for separating/recovering cells, which enables nucleated cells such as hemopoietic stem cells to be recovered in a high yield without using serum protein of a commercial preparation accompanying the fear of blood-transmitting infection.

This method features using, as a protein for the freeze preservation of cells, plasma obtained from blood in which the cells are contained, i.e., the so-called self-plasma. In the method for separating/recovering cells in which a cell-containing liquid comprising cells necessary for being recovered, cells to be removed and plasma is introduced into a cell-capturing means substantially capturing the cells necessary for being recovered and removably passing the cells to be removed and plasma, a removing cell-containing liquid is discharged from the cell-capturing means and the cells necessary for being recovered are recovered from the cell-capturing means by introducing an eluting liquid, the recovering procedure is performed while making the cell-containing liquid containing plasma remain in a small amount in the cell-capturing means.