To obtain the subject new vector comprising a vector, containing km/neo, pA-rep and pBR-ori and having a specific chain length and capable of efficiently transferring a gene by genetic manipulation of Amycolatopsis mediterranei.

This new cloning vector comprises a cloning vector pRLM10 of 7.4 kb, etc., containing km/neo, pA-rep and pBR-ori is used for genetic manipulation of Amycolatopsis mediterranei and capable of carrying out efficient transformation of a gene by electroporation. This cloning vector is obtained by digesting a pRL1 derivative by a restriction enzyme BamHI, digesting pIJ4026 by Bg1II, electrically eluting ermE gene, ligating the eluted ermE gene with a DNA containing the pRL1 derivative, then inserting the resultant ligation mixture into Escherichia coli, conducting the screening, isolating a strain containing pRL50 and pRL80 DNAs, transfecting the resultant strain into a microorganism of the genus Amycolatopsis and selecting the obtained vector under a selective pressure of an antibiotic substance.