Title:
生細胞又は生物学的活性因子を封入し送達するための、細胞適合性、注射可能、かつ自己ゲル化性のキトサン溶液の組成物
Document Type and Number:
Japanese Patent JP5670872
Kind Code:
B2
Abstract:
The present invention provides compositions and methods for tissue repair using a cytocompatible self-gelling cross-linked hydrogel. The composition comprises a biocompatible mixture of chitosan, bifunctional dialdehyde, and hydroxylated polymer, which can be used to immobilize or encapsulate viable cells, or bioactive substances. The method includes the process of mixing bioactive substances, live cells, and/or extracellular matrix components with a cross-linking solution comprising a bifunctional aldehyde-treated hydroxylated polymer such as hydroxyethyl cellulose. The cross-linking solution is then mixed homogenously with a neutral isotonic chitosan solution. The chitosan becomes cross-linked by the bifunctional aldehyde, while the cells are protected from potentially nocive effects of the aldehyde cross-linker by the hydroxylated polymer. The injectable solution retains cell viability and bioactivity, and immobilizes cells at the site of injection or delivery. Depending on the particular application, mixtures of chitosan and bifunctional dialdehyde may be employed. The injectable solution also liberates bioactive substances with controlled release kinetics from the site of injection.
Inventors:
ホーマン キャロライン
チェナイト アブデラティーフ
ブッシュマン マイケル
セレキ アレッシオ
サン ジュン
チェナイト アブデラティーフ
ブッシュマン マイケル
セレキ アレッシオ
サン ジュン
Application Number:
JP2011285187A
Publication Date:
February 18, 2015
Filing Date:
December 27, 2011
Export Citation:
Assignee:
ピラマル ヘルスケア （カナダ） リミテッド
International Classes:
A61K47/36; A61K9/06; A61K31/727; A61K31/728; A61K35/12; A61K35/14; A61K35/16; A61K38/00; A61K38/17; A61K38/22; A61K38/43; A61K39/12; A61K47/24; A61K47/34; A61K47/38; A61K48/00; A61L27/00; A61L27/20; A61L27/38; A61L27/50; A61L27/52; A61P3/02; A61P19/02; A61P19/08; A61P31/12; A61K9/00
Attorney, Agent or Firm:
Spring water Original aim
Masao Haruna
Hirotaka Yamaguchi
Shun Osakabe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Shin-ichi Watanabe
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Shun Osakabe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Shin-ichi Watanabe
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto