PURPOSE: To efficiently concentrate and separate the titled compound useful as a raw material for medicines, etc., without deterioration or loss of phospholipids, by dissolving a phospholipid fraction of a vegetable oil cake in a nonpolar solvent, bringing the resultant solution into contact with silica gel and eluting the fraction with a polar solvent, etc.

CONSTITUTION: A vegetable oil cake, e.g. soybean oil cake, etc., produced in purifying soybean oil, etc., is added to acetone, stirred and thoroughly dispersed. The formed supernatant is then removed and the acetone is removed to afford a phospholipid fraction, which is then dissolved in a nonpolar solvent, e.g. n- hexane, etc., passed through and brought into contact with a silica gel column and then eluted with a polar solvent or a mixture solution thereof with a nonpolar solvent to concentrate and separate a sterylglucoside and a fatty acid ester thereof into the same fraction or individual fractions.