Title:
DETECTION OF DNA SEQUENCE VARIATION
Document Type and Number:
Japanese Patent JP2006246897
Kind Code:
A
Abstract:
To provide a common method applicable to all variants generally used as a DNA marker for genetic analysis.
The analysis method comprises preparing a polynucleotide target involving nucleotide of a specified position and an oligonucleotide probe fixed on a support which probe complementary to the target and terminates at the specified position or its vicinity, and performing steps of (a) incubating the target with the probe to form a duplex chain, (b) incubating the duplex chain with a labelled oligonucleotide complementary to the target under a ligation condition and (c) monitoring the ligation of (b) as one exhibiting point mutation at the specific position in the target.
Inventors:
SOUTHERN EDWIN MELLOR
PRITCHARD CLARE E
CASE-GREEN STEPHEN C
PRITCHARD CLARE E
CASE-GREEN STEPHEN C
Application Number:
JP2006137603A
Publication Date:
September 21, 2006
Filing Date:
May 17, 2006
Export Citation:
Assignee:
OXFORD GENE TECH IP LTD
International Classes:
C12Q1/68; C12M1/00; C12N15/09; C12P19/34; C12Q1/6827; C12Q1/6834; C12Q1/6837; C12Q1/6858; C12Q1/6862; G01N33/53; G01N37/00; C12Q1/6869; C12Q1/6876
Attorney, Agent or Firm:
Kazuo Shamoto
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita