Title:
遺伝子組換え作物の検出方法
Document Type and Number:
Japanese Patent JP7091237
Kind Code:
B2
Abstract:
The genetically-modified crop detection method is for detecting a genetically-modified crop using a polymerase chain reaction, and comprises: a step for amplifying a nucleic acid having a partial sequence of a soybean-derived DREB gene, by using a nucleic acid in a sample to be tested or a nucleic acid extracted from the sample to be tested as a template, and a primer pair which can be used to specifically amplify the partial sequence of the soybean-derived DREB gene; and a step for detecting or quantifying the amplified nucleic acid. The present invention also provides a reagent kit for detecting or quantifying a genetically-modified crop having an introduced soybean-derived DREB gene using a polymerase chain reaction.
Inventors:
Satoshi Noma
Kikuchi Yosuke
Manami Nishi
Seiko Miyatake
Kikuchi Yosuke
Manami Nishi
Seiko Miyatake
Application Number:
JP2018507059A
Publication Date:
June 27, 2022
Filing Date:
January 12, 2017
Export Citation:
Assignee:
Nisshin Flour Milling Group Inc.
International Classes:
C12Q1/686; C12Q1/6858
Foreign References:
| CN1477109A | ||||
| CN1475497A |
Other References:
J.Agric.Food.Chem.,2013,Vol.61,p.10293-10301
Food Hyg.Saf.Sci.,2012,Vol.53,No.5,p.203-210
J.Agric.Food Chem.,2009,Vol.57,p.26-37
新農業展開ゲノムプロジェクト:DREB遺伝子等を活用した環境ストレスに強い作物の開発,新農業展開ゲノムプロジェクト,2014年,Vol.518,p.1-44
Glycine max dehydration responsive element binding protein(DREB1) mRNA,complete cds, database Genbank[online],2003年 6月 2日,AF514908
Gossypium hirsutum dehydration responsive element binding protein(DREB1) mRNA,complete cds, database Genbank[online],2003年 8月 1日,AF509502
Food Hyg.Saf.Sci.,2012,Vol.53,No.5,p.203-210
J.Agric.Food Chem.,2009,Vol.57,p.26-37
新農業展開ゲノムプロジェクト:DREB遺伝子等を活用した環境ストレスに強い作物の開発,新農業展開ゲノムプロジェクト,2014年,Vol.518,p.1-44
Glycine max dehydration responsive element binding protein(DREB1) mRNA,complete cds, database Genbank[online],2003年 6月 2日,AF514908
Gossypium hirsutum dehydration responsive element binding protein(DREB1) mRNA,complete cds, database Genbank[online],2003年 8月 1日,AF509502
Attorney, Agent or Firm:
Showa International Patent Office
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