Title:
ＦＫＰＡの精製、及び組換えポリペプチドの産生のためのその使用
Document Type and Number:
Japanese Patent JP7091238
Kind Code:
B2
Abstract:
The present invention provides methods for producing FKBP-type peptidyl-prolyl cis-trans isomerase (FkpA) polypeptides at very high levels of purity. Also provided we ultrapure FkpA and methods of using same, e.g., for use in immunoassays to show removal of FkpA from biologics produced in bacteria. In addition, the present invention provides methods of purifying polypeptides (e.g., multispecific antibodies) produced in bacteria overexpressing one or more chaperones. The methods include affinity chromatography, mixed-mode chromatography and hydrophobic interaction chromatography. in some aspects, the invention provides compositions of polypeptides (e.g., multispecific antibodies) that are essentially fee of product-specific impurities.
Inventors:
Fon, Chris Bee.
Radiwara, Asif
McKay, Patrick A.
Radiwara, Asif
McKay, Patrick A.
Application Number:
JP2018509536A
Publication Date:
June 27, 2022
Filing Date:
August 19, 2016
Export Citation:
Assignee:
Genentech, Incorporated
International Classes:
C07K16/46; C07K1/16; C07K1/18; C07K1/20; C07K1/22; C07K1/34; C07K1/36; C07K16/24
Domestic Patent References:
| JP2015071629A | ||||
| JP2015042645A | ||||
| JP2015501836A | ||||
| JP2013508387A |
Foreign References:
| WO2015070068A1 | ||||
| WO2014110246A1 |
Other References:
Journal of Chromatography A,2004年,Vo.1024, No.1-2,p.79-85
mAbs,2010年,Vol.2, No.5,p.480-499
Journal of Chromatography A,2012年,Vol.1221,p.57-70
mAbs,2010年,Vol.2, No.5,p.480-499
Journal of Chromatography A,2012年,Vol.1221,p.57-70
Attorney, Agent or Firm:
Sonoda/Kobayashi Patent Business Corporation
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