Title:
ヌクレオチドを細胞核内に送達するための装置および方法
Document Type and Number:
Japanese Patent JP3865318
Kind Code:
B2
Abstract:
The invention presents a system for inducing cells in living intact tissue, in vivo or ex vivo, to accept nucleotides from their extracellular environment and to localize those nucleotides into the cells' nuclei. This system relies on the fact that, when subjected to high pressure, cells take in nucleotides and localize those nucleotides into their nuclei with a transfection rate of greater than 90% in some cases. This invention employs various techniques for placing under high pressure either cells in isolated tissue cultures, or cells in tissues still connected to a living body. Such pressurization techniques include enclosing the tissue in an impermeable and inelastic sheath and injecting fluid into the tissue until a sufficiently high pressure is reached; placing a tissue culture in a dish and placing the dish in a pressurized chamber; mechanical pressurization techniques such as compression of a space containing fluids; occluding the ingress and egress of a vessel or organ to create a watertight compartment within the vessel or organ; and pressurization of an entire organism after delivery of nucleotide to the extracellular environment. This invention also employs various techniques for delivering a solution or suspension containing the nucleotide into the tissue; such techniques include the use of a syringe-like mechanism and a catheter.
Inventors:
Man Michael Jay.
Diet frank pee.
Jau Victor Jay.
Gibbons Garry H.
Vonder Rayen Heiko
Diet frank pee.
Jau Victor Jay.
Gibbons Garry H.
Vonder Rayen Heiko
Application Number:
JP53354496A
Publication Date:
January 10, 2007
Filing Date:
May 03, 1996
Export Citation:
Assignee:
The Board of Trustees of the Reland Stanford Junior University
International Classes:
C12N15/09; A61M5/00; A61M5/145; A61M25/00; A61M37/00; C12M1/00; C12M1/34; C12M3/00; C12N15/11; C12N15/113; C12N15/87; C12N15/89; A61F2/958
Domestic Patent References:
| JP3076568A |
Foreign References:
| WO1992004439A1 |
Other References:
Proc. Natl. Acad. Sci. USA, 1995 May, vol.92, p.4502-4506
Proc. Natl. Acad. Sci. USA, 1995 Feb., vol.92, p.1137-1141
Science, 1989, vol.244, p.1342-1344
Proc. Natl. Acad. Sci. USA, 1995 Feb., vol.92, p.1137-1141
Science, 1989, vol.244, p.1342-1344
Attorney, Agent or Firm:
Hatsushi Shimizu