To provide a technology effectively differentiation inducing biofunctional cells for tissue reproduction or restoration by using a stem cell separated form a biomedical tissue of mammal.
The technology comprises preparation of biofunctional cells for transplantation used for reproduction medicine with high taking rate by using stem cells separated from a biomedical tissue of mammal and combining an adhesion culture with a suspension culture. The cell aggregate (sphere body) with a size of 50-500 μm diameter on the suspension culture process is continuously kept in an undifferentiated state of the stem cells, and manifested an excellent improvement in differentiation inducing rate to biofunctional cells of the skin and taking rate after transplantation. The invention relates to the technology continuously keeping undifferentiated state of stem cells and then effectively inducing differentiation of biofunctional cells for reproduction or restoration of tissue with a high taking rate.
COPYRIGHT: (C)2008,JPO&INPIT
SAKAIDA TSUTOMU
NAKADA SATORU
AKAMATSU HIROHIKO
MATSUNAGA KAYOKO
YAMAMOTO NAOKI
MARUNOUCHI TOORU
| JP2005514944A | 2005-05-26 |