SYSTEM FOR IDENTIFYING AND SORTING LIVING CELLS

Japanese Patent JP2017012197

A

PROBLEM TO BE SOLVED: To generally provide cellular measurements based on mid-infrared absorption measurements.SOLUTION: In an embodiment of the present invention, a system and method of cytometry comprise the steps of: presenting a single sperm cell to at least one laser source configured to transfer light to the sperm cell in order to induce bond vibrations in the sperm cell DNA; and detecting the signature of the bond vibrations. The bond vibration signature is used to calculate a DNA content carried by the sperm cell which is used to identify the sperm cell as carrying an X-chromosome or Y-chromosome. Another system and method comprise the steps of: allowing cells to pass at least one QCL source one-by-one using a fluid treatment system; transferring QCL light to a single cell to induce resonant mid infrared absorption by one or more analytes of the cell; and detecting, using a mid-infrared detection facility, the transferred mid-infrared wavelength light, wherein the transferred mid-infrared wavelength light is used to identify a cell characteristic.SELECTED DRAWING: Figure 1

WAGNER MATTHIAS
JOHN HEANUE

JP2016198323A

January 19, 2017

October 06, 2016

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1087 SYSTEMS INC

C12M1/34; G01N21/3563; G01N33/48; G01N33/483; G01N33/50

JP2006524054A	2006-10-26
JP2007515936A	2007-06-21
JP2005530986A	2005-10-13
JP2000146819A	2000-05-26
JPH10512952A	1998-12-08
JP2009085872A	2009-04-23
JP2004520584A	2004-07-08
JP2013539983A	2013-10-31

US20050207943A1	2005-09-22
US20100248362A1	2010-09-30

Koichi Tsujii
Sadao Kumakura
Fumiaki Otsuka
Takaki Nishijima
Hiroyuki Suda
Hiroshi Uesugi
Yoshinori Kishi

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