Title:
EXPRESSION SYSTEM FOR STEM LOOP TYPE RNA MOLECULE HAVING RNAi EFFECT
Document Type and Number:
Japanese Patent JP2005046003
Kind Code:
A
Abstract:
To provide a new expression system for an RNA molecule in which expression of a target gene can be suppressed and to provide a method for preparing a knockdown cell using the system.
It is found that a stem loop type RNA molecule can efficiently be translocated into cytoplasm and RNi (RNA interference) effects can be exhibited by producing the RNA molecule from a DNA encoding the stem loop type RNA molecule utilizing a tRNA promoter. The RNAi effects can efficiently be exhibited even by transferring a cytoplasm translocation signal sequence into the DNA encoding the stem loop type RNA molecule. A cell in which a desired gene is knocked out can simply be prepared by using the expression system for the stem loop type RNA molecule.
Inventors:
TAHIRA KAZUMASA
KAWASAKI HIROAKI
KAWASAKI HIROAKI
Application Number:
JP2003121699A
Publication Date:
February 24, 2005
Filing Date:
April 25, 2003
Export Citation:
Assignee:
GENOFUNCTION INC
NAT INST OF ADV IND & TECHNOL
TAHIRA KAZUMASA
KAWASAKI HIROAKI
NAT INST OF ADV IND & TECHNOL
TAHIRA KAZUMASA
KAWASAKI HIROAKI
International Classes:
C12N1/19; C12N5/10; C12N15/09; A01K67/027; (IPC1-7): C12N15/09; A01K67/027; C12N1/19; C12N5/10
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto
Kazunori Hashimoto