To provide a fluorescence microscope device achieving selective visual field-observation of aimed fluorescence.
The fluorescence microscope device includes: an optical microscope 3 which irradiates a sample 16 including a phosphor 1 whose fluorescence intensity is varied by magnetic resonance with exciting light to observe the fluorescence of the phosphor 1; a high-frequency magnetic field generation part 5 which irradiates the sample 16 with high-frequency magnetic field for generating electron spin magnetic resonance; a modulation part 7 which generates a modulation signal for modulating the high-frequency magnetic field; a detector 9 which detects light intensity on a surface of the sample observed by the optical microscope 3 in each of a plurality of pixels at each sampling time while modulating the high-frequency magnetic field; and a processing unit 10 which extracts an object pixel in which time-sequential variation of the light intensity correlates with the modulation signal from the plurality of pixels.
HARADA YOSHISHIGE
YOKOTA HIROAKI
YOSHINARI YOSUKE
TSUJI SEIGO
JEOL LTD
| JPH04292782A | 1992-10-16 | |||
| JP2001147160A | 2001-05-29 | |||
| JPH04295782A | 1992-10-20 |
JPN6013058879; Ｇ． Ｂａｌａｓｕｂｒａｍａｎｉａｎ ｅｔ ｅｌ．: '"Ｎａｎｏｓｃａｌｅ ｉｍａｇｉｎｇ ｍａｇｎｅｔｏｍｅｔｒｙ ｗｉｔｈ ｄｉａｍｏｎｄ ｓｐｉｎｓ ｕｎｄｅｒ ａｍｂｉｅｎｔ ｃｏｎｄｉｔｉｏｎｓ"' ｎａｔｕｒｅ Ｖｏｌ． ４５５, 20081002, ｐ． ６４８-６５２
Iwa Saki Kokuni
Masakazu Ito
Shunichi Takahashi
Toshio Takamatsu