GENE EXPRESSION ANALYSIS METHOD USING TWO-DIMENSIONAL CDNA LIBRARY

Title:

GENE EXPRESSION ANALYSIS METHOD USING TWO-DIMENSIONAL CDNA LIBRARY

Document Type and Number:

Japanese Patent JP2014027955

Kind Code:

A

Abstract:

To provide a method and/or means for picking out one cell and analyzing it and for quantitatively monitoring expression level of various genes with maintaining two-dimensional information of a tissue.

The invention provides a method for obtaining gene expression level in any loci or all of the target loci at one cell level positional resolution by producing cDNA library from mRNA with maintaining two-dimensional cell distribution information in a living tissue. More concretely, sheet shape cDNA library is produced from mRNA with maintaining two-dimensional cell distribution information in the living tissue and this is repeatedly used in gene expression detection steps, thereby realizing a high precision expression distribution measurement for a large number of genes.

Inventors:

SHIRAI MASATAKA
KANBARA HIDEKI
TANIGUCHI KIYOMI

Application Number:

JP2013234715A

Publication Date:

February 13, 2014

Filing Date:

November 13, 2013

Export Citation:

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Assignee:

HITACHI LTD

International Classes:

C12M1/00; C40B40/08; C12Q1/68

Domestic Patent References:

JP2003505694A	2003-02-12
JP2007014297A	2007-01-25
JP2003526331A	2003-09-09

Foreign References:

WO2011068088A1	2011-06-09
WO2011068088A1	2011-06-09

Attorney, Agent or Firm:

Yusuke Hiraki
Satoshi Fujita

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