グリコシル化プロファイル分析 - F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT

Title:

グリコシル化プロファイル分析

Document Type and Number:

Japanese Patent JP2010536355

Kind Code:

A

Abstract:

The present invention provides a method for the production of a glycosylated heterologous polypeptide comprising the steps of obtaining a sample from a crude fermentation broth, incubation of the sample with magnetic affinity beads, releasing glycans from the immobilized glycosylated polypeptides, measuring a glycosylation profile, comparing the glycosylation profile with a desired glycosylation profile of the recombinant glycosylated polypeptide, modifying the culture conditions in accordance to the glycosylation profile obtained, and repeating the process in order to obtain a glycosylated heterologous polypeptide with the desired glycosylation profile. With a similar method diagnostic markers can be identified and quantified.

Inventors:

Harberger, Marx
Royce, Ditmer

Application Number:

JP2010521356A

Publication Date:

December 02, 2010

Filing Date:

August 20, 2008

Export Citation:

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Assignee:

F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT

International Classes:

C12N15/00; C12P21/02; G01N27/62; G01N30/06; G01N30/08; G01N30/72; G01N30/88; G01N33/53

Domestic Patent References:

JP2006515927A

2006-06-08

Foreign References:

WO2006133148A2	2006-12-14
WO2007035930A2	2007-03-29

Other References:

JPN5010011087; ＳＫＩＢＥＬＩ　ＶＥＮＫＥ: 'Ｓｕｇａｒ　ｐｒｏｆｉｌｉｎｇ　ｐｒｏｖｅｓ　ｔｈａｔ　ｈｕｍａｎ　ｓｅｒｕｍ　ｅｒｙｔｈｒｏｐｏｉｅｔｉｎ　ｄｉｆｆｅｒｓ　ｆｒｏｍｒｅｃｏｍｂｉｎａｎｔ　ｈｕｍａｎ　ｅｒｙｔｈｒｏｐｏｉｅｔｉｎ．' ＢＬＯＯＤＶ９８　Ｎ１３, 20011215, Ｐ３６２６-３６３４, Ｗ．Ｂ．　ＳＡＵＮＤＥＲＳ　ＣＯＭＰＡＮＹ
JPN5010011089; ＰＡＰＡＣ　Ｄ　Ｉ: 'Ａ　ｈｉｇｈ-ｔｈｒｏｕｇｈｐｕｔ　ｍｉｃｒｏｓｃａｌｅ　ｍｅｔｈｏｄ　ｔｏ　ｒｅｌｅａｓｅ　Ｎ-ｌｉｎｋｅｄ　ｏｌｉｇｏｓａｃｃｈａｒｉｄｅｓ　ｆｒｏｍｇｌｙｃｏｐｒｏｔｅｉｎｓ　ｆｏｒ　ｍａｔｒｉ' ＧＬＹＣＯＢＩＯＬＯＧＹＶ８　Ｎ５, 199805, Ｐ４６３-４７２, ＩＲＬ　ＰＲＥＳＳ

Attorney, Agent or Firm:

Hajime Tsukuni
Fusayuki Saito

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