Title:
Heat-prone exonuclease
Document Type and Number:
Japanese Patent JP6316505
Kind Code:
B2
Abstract:
The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.
Inventors:
Solstad, Therese
Andre Assen, Elizabeth, Lill
Lorenzen, Maritt, Sujo
Rains, Olaf
Erde, Morten
Larsen, Atre, Noralph
Piotrovsky, Yvonne
Willassen, Nils, Pedder
Andre Assen, Elizabeth, Lill
Lorenzen, Maritt, Sujo
Rains, Olaf
Erde, Morten
Larsen, Atre, Noralph
Piotrovsky, Yvonne
Willassen, Nils, Pedder
Application Number:
JP2017519228A
Publication Date:
April 25, 2018
Filing Date:
August 19, 2015
Export Citation:
Assignee:
ARCTICZYMES AS
Unibersitet Itromso-Norges Arcticus Unibersitet
Unibersitet Itromso-Norges Arcticus Unibersitet
International Classes:
C12N15/09
Domestic Patent References:
| JP2005531314A | ||||
| JP2011524738A | ||||
| JP6165678A |
Attorney, Agent or Firm:
Patent corporation ssinpat
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