Title:
高親和性コリントランスポーター
Document Type and Number:
Japanese Patent JP4018304
Kind Code:
B2
Abstract:
The present invention provides a protein having high-affinity choline transporter activity which is important physiologically, a gene encoding the protein, and a method of screening a material promoting the high-affinity choline transporter activity with the use of the same, and the like. By examining high-affinity choline uptake activity of Na<+>-dependent transporter cDNA deduced from the genomic sequence of a nematode (C.elegans) in a Xenopus oocyte expression system, the cDNA (cho-1) of nematode high-affinity choline transporter is identified. Then the cDNA (CHT1) of rat high-affinity choline transporter is cloned from rat spinal cord by using the homology of a base sequence to this cDNA as an index. Similarly, the cDNA of human high-affinity choline transporter is cloned from human genome.
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Inventors:
Tatsuya Haga
Takashi Okuda
Takashi Okuda
Application Number:
JP36899199A
Publication Date:
December 05, 2007
Filing Date:
December 27, 1999
Export Citation:
Assignee:
Japan Science and Technology Agency
International Classes:
A01K67/027; C07K14/47; A61K38/00; C12N15/09; A61P25/28; C07K14/435; C07K14/705; C07K16/18; C07K19/00; C12N5/10; C12N15/12; C12P21/02; C12P21/08; C12Q1/00; G01N33/50; G01N33/68; A61K48/00
Other References:
Biochimica et Biophysica Acta,1991年,Vol.1065, No.2,p.107-113
Annals New York Academy of Science,1996年,Vol.777,p.197-204
J. Clin. Invest.,1994年,Vol.97, No.2,p.696-702
Annals New York Academy of Science,1996年,Vol.777,p.197-204
J. Clin. Invest.,1994年,Vol.97, No.2,p.696-702
Attorney, Agent or Firm:
Masaki Hirota