Title:
HIGH LEVEL EXPRESSION OF PROTEIN IN YEAST
Document Type and Number:
Japanese Patent JP2004081222
Kind Code:
A
Abstract:
To provide a method and material for increasing yield of a foreign protein.
This invention provides a method of expressing a non-yeast protein under the control of ADH (alcohol dehydrogenase) 2 control sequence in an yeast host having enhanced ADRI expression, and a transformed yeast host. Provided is a transformed yeast which has an expression cassette incorporated in a genome containing a coding sequence of ADRI under the control of a transcription initiation sequence and a transcription termination sequence recognized by the yeast and the transcription initiation sequence is a promotor except an ADR1 promotor.
Inventors:
SHUSTER JEFFREY R
Application Number:
JP2003412625A
Publication Date:
March 18, 2004
Filing Date:
December 10, 2003
Export Citation:
Assignee:
CHIRON CORP
International Classes:
C12N1/19; C07K14/50; C12N15/09; C12N15/81; C12N15/90; C12P21/00; C12R1/865; (IPC1-7): C12N15/09; C12N1/19
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita