Title:
HYBRIDIZATION METHOD
Document Type and Number:
Japanese Patent JP3454847
Kind Code:
B2
Abstract:
PURPOSE: To prevent the self-hybridization of a specimen nucleic acid and improve the detection sensitivity of a gene detection process.
CONSTITUTION: A nucleic acid used as a specimen is hybridized with a nucleic acid probe containing a sequence complementary to a part of the objective gene sequence to be detected. The hybridization is carried out by adding a nucleic acid chain composed of a sequence complementary to the sequence which is not complementary to the nucleic acid probe in the objective gene sequence together with the nucleic acid probe.
Inventors:
Koji Hashimoto
Keiko Miwa
Masashiki Goto
Yoshio Ishimori
Keiko Miwa
Masashiki Goto
Yoshio Ishimori
Application Number:
JP22718992A
Publication Date:
October 06, 2003
Filing Date:
August 26, 1992
Export Citation:
Assignee:
Toshiba Corporation
International Classes:
C12Q1/68; C12N15/09; G01N27/416; (IPC1-7): C12Q1/68
Domestic Patent References:
| JP484899A | ||||
| JP2291300A | ||||
| JP4141099A | ||||
| JP62188970A |
Attorney, Agent or Firm:
Takehiko Suzue