Title:
IDIOSYNCRATICALLY CUTTING LINKER
Document Type and Number:
Japanese Patent JPS56166200
Kind Code:
A
Abstract:
The present invention is based upon a general principle of providing specific olingonucleotide segments (<>, herein) to be attached in sequence to a cloned DNA coding segment. The linkers of the present invention confer desired functional properties on the expression of the protein coded by the coding sequence. Using linkers of the present invention, the desired protein may be expressed either as a fusion or non-fusion protein. A linker coding for an additional sequence of amino acids may be attached, the sequence being chosen to provide properties exploitable in a simplified purification process. A linker coding for an amino acid sequence of the extended specific cleavage site of a proetolytic enzyme is provided, as well as specific cleavage linkers for simple specific cleavage sites.
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Inventors:
UIRIAMU JIEI RUTSUTAA
Application Number:
JP2929481A
Publication Date:
December 21, 1981
Filing Date:
February 28, 1981
Export Citation:
Assignee:
UNIV CALIFORNIA
International Classes:
C07H21/00; C07H21/04; C07K14/62; C12N15/09; C12N15/00; C12N15/62; C12N15/66; C12N15/70; C12P21/00; C12R1/01; (IPC1-7): C07H21/00; C12N15/00; C12P21/00
Domestic Patent References:
| JPS54163600A | 1979-12-26 | |||
| JPS5519092A | 1980-02-09 | |||
| JPS5621596A | 1981-02-28 |