Title:
植物エキスの皮膚感作性インビトロ評価法
Document Type and Number:
Japanese Patent JP7110825
Kind Code:
B2
Abstract:
To provide a method for evaluating skin sensitization to plant extract that has not been detected so far in an in-vitro evaluation system of a sensitizing substance using a cell.SOLUTION: The in-vitro evaluation method or screening method of a sensitizing substance, adding and exposing a test object that is supposed to include a sensitizing substance in a cell to detect a molecule expressed on a surface of he cell, is characterized in that the test object that is supposed to include the sensitizing substance is a plant extract, a concentration of the plant extract to be added is 5000 μg/ml-500000 μg/ml, and an exposure time is 1-30 minutes. The in-vitro evaluation method includes a process of a skin sensitization evaluation method to the plant extract without using an animal, which has been hard to evaluate so far.SELECTED DRAWING: Figure 2
Inventors:
Yuzu Yuzu Matsuoka
Toru Nagahama
Yoshimura Tomohisa
Hiroshi Itagaki
Toru Nagahama
Yoshimura Tomohisa
Hiroshi Itagaki
Application Number:
JP2018156842A
Publication Date:
August 02, 2022
Filing Date:
August 24, 2018
Export Citation:
Assignee:
Taisho Pharmaceutical Co., Ltd.
International Classes:
G01N33/50; C12Q1/02; G01N33/15; G01N33/53
Domestic Patent References:
| JP2010504744A | ||||
| JP2001221796A | ||||
| JP2004222582A | ||||
| JP10232225A |
Foreign References:
| WO2010024221A1 | ||||
| US20140213466 |
Other References:
MIZOGUCHI, I. et al.,Prediction of Chemical Respiratory and Contact Sensitizers by OX40L Expression in Dendritic Cells Using a Novel 3D Coculture System,Frontiers in Immunology,2017年08月,Vol.8, Article 929,pp.1-12
SHIRAISHI, E. et al.,Utility of murine dendritic cell line DC2.4 for in vitro assay of skin-sensitization potential,Fundamental Toxicological Sciences,2017年06月09日,Vol.4, No.3,pp.121-126
SHIRAISHI, E. et al.,Utility of murine dendritic cell line DC2.4 for in vitro assay of skin-sensitization potential,Fundamental Toxicological Sciences,2017年06月09日,Vol.4, No.3,pp.121-126