Title:
IN VITRO MATURATION OF DENUDED BOVINE OOCYTE
Document Type and Number:
Japanese Patent JPH06217765
Kind Code:
A
Abstract:
PURPOSE: To provide a process for in vitro maturation of denuded bovine oocytes.
CONSTITUTION: This process of maturing a denuded bovine oocytes comprises: a. isolating bovine cumulus oocyte complexes; b. placing the complexes in a maintenance medium; c. removing the cumulus cells from the oocytes to form denuded bovine oocytes; and d. maturing the denuded oocytes in a conditioned medium containing a monolayer of cumulus cells for about 10-24 hours, preferably for 16-20 hours. In addition, this process can be applied to the method for screening the oocytes suitable for fertilization and embryo growth. This process includes the observation of growth and excretion of the polar body.
Inventors:
TANJIYA DOMINKO
Application Number:
JP17453992A
Publication Date:
August 09, 1994
Filing Date:
July 01, 1992
Export Citation:
Assignee:
TANJIYA DOMINKO
International Classes:
A01K67/02; C12N5/075; C12N15/09; (IPC1-7): C12N5/06; A01K67/02
Attorney, Agent or Firm:
Tadahiko Ito (1 outside)