Title:
骨組織からの精製TGF-1およびTGF-2の単離
Document Type and Number:
Japanese Patent JP2004528279
Kind Code:
A
Abstract:
A method for purifying bone-derived TGF-beta proteins including an anion exchange process, a cation exchange process, and a reverse phase HPLC process, and optionally, a filtration process, a Heparin-Sepharose process, and/or a reverse phase HPLC desalting process. The filtration process preferably selects proteins having a nominal molecular weight between approximately 10 kilodaltons and approximately 100 kilodaltons. Preferably, the anion exchange process uses a strongly cationic resin having quaternary amine functional groups. Preferably, the cation exchange process uses a strongly anionic resin having sulfonic acid functional groups. The TGF-beta proteins can be eluted from the reverse phase HPLC column with an acetonitrile solution in combination with aqueous trifluoracetic acid. The purification processes yield highly enriched TGF-beta1 and TGF-beta2 proteins.
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Inventors:
Junker, Lewis
Louis, michael
Robert Nelson
Louis, michael
Robert Nelson
Application Number:
JP2002552005A
Publication Date:
September 16, 2004
Filing Date:
December 18, 2001
Export Citation:
Assignee:
SULZER BIOLOGICS INC.
International Classes:
A61K38/00; A61P17/02; A61P43/00; C07K1/14; C07K1/18; C07K1/20; C07K1/34; C07K14/495; (IPC1-7): C07K14/495; A61K38/00; A61P17/02; A61P43/00; C07K1/14; C07K1/18; C07K1/20; C07K1/34
Attorney, Agent or Firm:
Yanagita Seiji
Go Sakuma
Go Sakuma