Title:
微少流体を用いる白血球および微小粒子分画
Document Type and Number:
Japanese Patent JP6757981
Kind Code:
B2
Abstract:
This invention relates to a method for separating blood cells comprising the steps of: (a) lysing red blood cells of a blood sample and diluting said sample; (b) providing a microfluidic device comprising a spiral-shaped flow channel having at least a first end and a second end, wherein said flow channel has two inlet ports at or near said first end and at least two outlet ports at or near said second end, wherein one of the two inlet ports is located at the inner wall of the spiral-shaped flow channel and the other inlet port is located at the outer wall of the spiral-shaped flow channel and at least one of the outlet ports is connected to a container allowing the storage of blood cells; (c) introducing the sample of step (a) into the inlet port located at the outer wall of the spiral-shaped flow channel and introducing a sheath fluid into the inlet port located at the inner wall of the spiral-shaped flow channel; (d) driving said sample and the sheath fluid through the spiral-shaped flow channel; and (e) recovering the blood cell in the at least one container connected to the at least one outlet port. The present invention also relates to coupling above described method of purifying neutrophils in the native state with a method for diagnosing diabetes or an inflammatory disease in a subject, which involves further investigation of neutrophils by determining their rolling speed, the neutrophil circularity (NC) index and/or the expression of markers, such as intracellular reactive oxygen species (ROS), CD1 1 b or PSGL-1.
Inventors:
Hou, Han Way
Behm, bernhard otto
Tay, female
Rho, Seichai Joachim
Behm, bernhard otto
Tay, female
Rho, Seichai Joachim
Application Number:
JP2017567746A
Publication Date:
September 23, 2020
Filing Date:
June 30, 2016
Export Citation:
Assignee:
Nanyang Technological University
International Classes:
G01N35/08; G01N33/53; G01N37/00
Domestic Patent References:
| JP2013521001A | ||||
| JP2015058394A | ||||
| JP2010538241A | ||||
| JP2013500012A |
Foreign References:
| WO2014152643A2 | ||||
| US20110070581 | ||||
| WO2014046621A1 | ||||
| WO2015057159A1 | ||||
| US8208138 | ||||
| WO2013085797A1 | ||||
| CN103923825A |
Other References:
Nivedita Nivedita,Continuous separation of blood cells in spiral microfluidic devices,BIOMICROFLUIDICS,2013年 9月 5日,Vol. 7, 054101,pp. 1-13
Attorney, Agent or Firm:
Makoto Onda
Hironobu Onda
Atsushi Honda
Miki Nakamura
Hironobu Onda
Atsushi Honda
Miki Nakamura