To provide a method for detecting, quantifying and identifying 16SrRNA gene, RNA or DNA probe, and petroleum-digesting bacterium.
The method of detecting and quantifying a microorganism having a specific function and its gene from natural environment involves: (1) a step of estimating the predominance of the microorganism having a specific function in the natural environment; (2) a step of amplifying specific gene domains of the microorganism having a specific function in a liquid culture medium of the highest dilution ratio at which the proliferation of the microorganism is judged as positive, followed by cloning; (3) a step of examining the difference among the gene domains thus cloned and determining the base sequences thereof; and (4) a step of identifying the microorganism having a specific function inhabiting in the natural environment from the base sequence data thus determined. 16SrDNAs having a specific base sequence are provided. RNA or DNA probes of 10 bp to 50 bp in size, which have a part of a specific base sequence and are hybridizable specifically with a petroleum-digesting bacterium belonging to the genus Cycloclasticus, are provided and use thereof is provided.
HIGASHIHARA TAKANORI
ISHIWATARI HIROYUKI
FUJITA TSUNEMI
NISHIMATSU CONSTR CO LTD
MTI KK
JPN6010034990, Ｄａｔａｂａｓｅ ＤＤＢＪ／ＥＭＢＬ／ＧｅｎＢａｎｋ ［ｏｎｌｉｎｅ］， Ａｃｃｅｓｓｉｏｎ Ｎｏ． Ｌ３４９５５, "＜ｈｔｔｐ：／／ｗｗｗ．ｎｃｂｉ．ｎｌｍ．ｎｉｈ．ｇｏｖ／ｓｖｉｅｗｅｒ／ｖｉｅｗｅｒ．ｆｃｇｉ？ｖａｌ＝６３２９１３＆ｓａｔ＝ＯＬＤＩＤ＆ｓａｔｋｅｙ＝１１６７１７７＞", ２０−ＪＡＮ−１９９５ｕｐｌｏａｄｅｄ， Ｄｙｋｓｔｅｒｈｏｕｓｅ，Ｓ．Ｅ． ｅｔ ａｌ．
JPN6010034991, Ａｐｐｌ． Ｅｎｖｉｒｏｎ． Ｍｉｃｒｏｂｉｏｌ．, 199609, Ｖｏｌ．６２， Ｎｏ．９, ｐｐ．３３４４−３３４９
JPN6010034992, Ａｐｐｌ． Ｅｎｖｉｒｏｎ． Ｍｉｃｒｏｂｉｏｌ．, 199812, Ｖｏｌ．６４， Ｎｏ．１２, ｐｐ．４７０３−４７１０
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