Title:
全血中におけるトロンビン活性の測定
Document Type and Number:
Japanese Patent JP4897794
Kind Code:
B2
Abstract:
The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of:
- contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm 2 ;
- allowing thrombin to generate in said sample;
- measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.
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Inventors:
Hemkel, Hendrick Coenrard
Beguin, Suzette
Al-Dieri, Raed
Wagenfold, Robert
News, Sabbathearn
Giesen, peter
Beguin, Suzette
Al-Dieri, Raed
Wagenfold, Robert
News, Sabbathearn
Giesen, peter
Application Number:
JP2008508173A
Publication Date:
March 14, 2012
Filing Date:
April 26, 2006
Export Citation:
Assignee:
SYNAPSE B.V.
International Classes:
G01N21/78; C12Q1/02; C12Q1/37; G01N33/86
Domestic Patent References:
| JP7506429A |
Foreign References:
| WO2003093831A1 | 2003-11-13 |
Attorney, Agent or Firm:
Satoshi Kono
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Toshio Shirane
Sadao Muramatsu
Nobuhisa Nogawa
Katsu Sunagawa
Ryo Hashimoto
Tetsuya Kazama
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Toshio Shirane
Sadao Muramatsu
Nobuhisa Nogawa
Katsu Sunagawa
Ryo Hashimoto
Tetsuya Kazama