MEMBRANE PROTEIN EXPRESSION SYSTEM AND ITS APPLICATION IN DRUG SCREENING

Japanese Patent JP2010011851

A

To provide a convenient in vitro membrane protein expression system using an inhibitor of drug efflux pump proteins and heterologous cells for testing other drug resistance-relating cell membrane proteins.

Provided is a protein expression system comprising an in vitro cell-based expression system for over-expression of a heterologous protein having efflux pump action relating to the drug resistance of a host cell membrane for drug screening applications, that is to say, a protein expression system comprising: (i) a host yeast cell; and (ii) a vector comprising the coding sequence of a target heterologous membrane protein, wherein the sequence is under the control of a promoter which, upon transformation of the host cell and chromosomal integration, causes over-expression of the functional target protein in the membrane of the host cell.

MONK BRIAN CHARLES
CANNON RICHARD DAVID
NAKAMURA KENJIRO
NIIMI SHOICHI
NIIMI KYOKO
HOLMES ANN RACHEL
LAMPING ERWIN
HARDING DAVID ROGER KAY
GOFFEAU ANDRE
DECOTTIGNIES ANABELLE

JP2009176778A

January 21, 2010

July 29, 2009

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UNIV OF OTAGO
UNIV CATHOLIQUE LOUVAIN
UNIV MASSEY

C12N15/09; A01N63/00; G01N33/50; A01P3/00; A61K31/4196; A61K38/00; A61K45/00; A61P31/04; A61P31/10; A61P43/00; C07K14/37; C07K14/40; C07K14/435; C07K14/705; C12N1/19; C12N15/81; C12P21/02; C12Q1/02; C12Q1/18; G01N33/15; G01N33/566

JPN6008040412; 歯基礎誌 第４２巻５号（抄録集）, 2000, 第１６８頁
JPN6008040414; 歯界展望 第９３巻第６号, 1999, 第１３６８頁-１３７６頁
JPN6008040416; Ｊａｐａｎｅｓｅ　Ｊ．　Ｍｅｄｉｃａｌ　Ｍｙｃｏｌｏｇｙ，　（１９９８），　Ｖｏｌ．　３９，　Ｎｏ．　２，　ｐ．　７３-７８
JPN6008040418; Ｊ．　Ｂｉｏｌ．　Ｃｈｅｍ．，　（１９９８），　Ｖｏｌ．　２７３，　Ｎｏ．　２０，　ｐ．　１２６１２-１２６２２

Yoshiyuki Inaba
Toshifumi Onuki

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