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Title:
METHOD AND APPARATUS FOR INSPECTING ULCERATIVE COLITIS, AND SCREENING METHOD OF THERAPEUTIC DRUG
Document Type and Number:
Japanese Patent JP2021072799
Kind Code:
A
Abstract:
To provide a method for inspecting ulcerative colitis which is useful for determining a disease state of ulcerative colitis.SOLUTION: A method for producing a strain sample derived from a subject in which the strain diversity and/or the number of strains in the sample of the subject are maintained includes: 1) a process of adding the sample of the subject to a culture medium; 2) a process of performing an operation to bring pH of the culture medium to 6.2 to 6.7 in starting the cultivation if necessary; and 3) a process of culturing the sample after starting the cultivation without performing an operation intended to maintain pH of the culture medium. Preferably, the method includes A) a process of performing sterilization of the culture medium and aeration to anaerobic gas and/or B) a process of suspending the sample to ascorbic acid-added phosphate buffer, before 1).SELECTED DRAWING: Figure 1

Inventors:
SASAKI KENGO
HOSHI NAMIKO
INOUE JUN
AZUMA TAKESHI
OSAWA AKIRA
SASAKI DAISUKE
KONDO AKIHIKO
Application Number:
JP2021002238A
Publication Date:
May 13, 2021
Filing Date:
January 08, 2021
Export Citation:
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Assignee:
UNIV KOBE
International Classes:
C12Q1/02; C12N1/20; C12Q1/24; G01N33/15; G01N33/50; G01N33/84
Foreign References:
WO2015136916A12015-09-17
Other References:
日消外会誌, vol. 16, no. 3, JPN6020040831, 1983, pages 552 - 556, ISSN: 0005017005
MSPHERE, vol. Vol. 2, Issue 3, e00047-17, JPN7023001173, 2017, ISSN: 0005017006
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takeshi Komatani
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto