PURPOSE: To measure isocitric acid or α-ketoglutaric acid in a specimen in high sensitivity and in high precision with a small amount of the specimen by employing a reagent comprising an isocitric acid dehydrogenase and two kinds of specific coenzymes.
CONSTITUTION: A specimen containing isocitric acid and/or α-ketoglutaic acid is treated with a reagent containing (A) thionicotine amide adenine dinucleotide (phosphate) [thin NAD(P)] and NAD(P) as coenzymes and an isocitric acid dehydrogenase catalyzing a reverse reaction producing α-ketoglutaric acid and CO2 from the isocitric acid, (B) the thio NAD(P) or the NAD(P), and (C) reduction type NAD(P) when the component B is the thio NAD(P), or reduction type thio NAD(P) when the component B is the NAD(P), to subject the specimen to a cyclic reaction of the reaction formula (A1, B1 A2 and B2 are each the reduction products of B1 C, A1 and B1, respectively), followed by measuring the conversion of A2 or B1.
TAKAHASHI MAMORU
MISAKI HIDEO
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