METHOD FOR CULTIVATING CELL AND APPARATUS THEREFOR

Title:

METHOD FOR CULTIVATING CELL AND APPARATUS THEREFOR

Document Type and Number:

Japanese Patent JPS60232088

Kind Code:

A

Abstract:

PURPOSE: To disperse cells uniformly in a liquid culture medium, by rotating a cultivation vessel on the horizontal axis thereof, and moving particles to be dispersed or suspended multiplicative cells in the mixing system.

CONSTITUTION: A liquid culture medium 3 containing cells 2 added thereto is filled in a cylindrical closed cultivation vessel 1, which is rotated on a shaft (X) at a constant rotational speed. The cells 2 change the existing position relative to the outside of the cultivation vessel 1 by the viscosity of the liquid culture medium 3 while scarcely changing the relative position in the liquid culture medium 3. Gravity acts on the cells 2 from different directions one after another to disperse the cells 2 in the liquid culture medium 3 with high dispersibility.

Inventors:

TOZAKI CHIKAO
YAMADA KEIICHI
KARIYA MASAO

Application Number:

JP8834584A

Publication Date:

November 18, 1985

Filing Date:

May 04, 1984

Export Citation:

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Assignee:

JAPAN SYNTHETIC RUBBER CO LTD

International Classes:

C12M1/12; C12M3/02; C12N5/02; C12N5/07; C12N5/071; (IPC1-7): C12M3/02; C12N5/02

Domestic Patent References:

JPS61177981A	1986-08-09
JPS58134989A	1983-08-11

Attorney, Agent or Firm:

Masahiko Oi

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