Title:
METHOD FOR DETECTING AND AMPLIFYING NUCLEIC ACID SEQUENCE USING MODIFIED OLIGONUCLEOTIDE HAVING INCREASED TARGET SPECIFIC TM
Document Type and Number:
Japanese Patent JP2008283973
Kind Code:
A
Abstract:
To provide oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence, and to provide a method for using these modified oligonucleotides.
The modified oligonucleotides include oligonucleotides containing at least one 2'-O-methylribofuranosyl moiety joined to a nitrogenous base. The oligonucleotides can be modified to preferentially bind RNA targets. These oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence.
Inventors:
BECKER MICHAEL MCCLELLAN
MAJLESSI MEHRDAD
MAJLESSI MEHRDAD
Application Number:
JP2008137832A
Publication Date:
November 27, 2008
Filing Date:
May 27, 2008
Export Citation:
Assignee:
GEN PROBE INC
International Classes:
C12N15/09; C12Q1/68; C07H21/00; C12Q1/6832; C12Q1/686; C12Q1/6865; G01N33/53
Domestic Patent References:
| JPH0866200A | 1996-03-12 |
Attorney, Agent or Firm:
Kazuo Shamoto
Shosuke Imai
Yasushi Kobayashi
Reiko Izumiya
Shosuke Imai
Yasushi Kobayashi
Reiko Izumiya