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Title:
METHOD FOR DETECTING GENES
Document Type and Number:
Japanese Patent JP2009175039
Kind Code:
A
Abstract:

To provide a method for electrochemically detecting genes, capable of detecting the hybridization of an target gene and a nucleic acid probe, in a time which is shorter than that of the electrochemical method by using an intercalator, and with a safety process which is further simplified than that of the method.

The method for detecting genes includes the steps of: inserting a gold electrode with a single-stranded nucleic acid probe fixed to a surface thereof to an electrolyte solution containing an target gene, which has a base sequence complementary to the target gene; performing galvanic deposition of copper ions with the gold electrode as a working electrode, after the addition of copper ions; checking a change in the galvanic deposition phenomenon by hybridization of the nucleic acid probe and the target gene; and then detecting the target gene.


Inventors:
SAWAGUCHI TAKAHIRO
IIJIMA SEIICHIRO
SATO YASU
NIWA OSAMU
Application Number:
JP2008015066A
Publication Date:
August 06, 2009
Filing Date:
January 25, 2008
Export Citation:
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Assignee:
NAT INST OF ADV IND & TECHNOL
International Classes:
G01N27/416; C12Q1/68; G01N27/327; G01N27/48; G01N33/53; G01N33/543; G01N37/00; C12N15/09
Domestic Patent References:
JP2003344345A2003-12-03
Foreign References:
WO2007116811A12007-10-18