Title:
METHOD FOR DETECTING MONOBASIC SUBSTITUTION BY FLUORESCENCE CORRELATION SPECTROSCOPY
Document Type and Number:
Japanese Patent JP3910000
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for detecting a monobasic substitution by which a high-sensitive and high-accuracy detection of the monobasic substitution can simply be carried out, the simple method for detecting the monobasic substitution without requiring B/F separation, polymerase chain reaction(PCR), cataphoresis, etc., and further the method for detecting the monobasic substitution by which plural polymorphic sites can simultaneously be detected if required by using small amounts of a specimen and a reagent.
SOLUTION: This method of detection is for detecting the monobasic substitution and comprises (1) hybridizing a specimen DNA containing the monobasic substitution site and at least one kind of DNA probe having a complementary sequence to a saquence anticipated in being included in the specimen DNA and having a labeled substance labeled on the base correspond to the monobasic substitution site and (2) optically assaying a positional variation of the labeled substance at plural lapse points in the progress of the hybridization.
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Inventors:
Kunio Hori
Application Number:
JP2000087501A
Publication Date:
April 25, 2007
Filing Date:
March 27, 2000
Export Citation:
Assignee:
Olympus Endo Technology America Inc.
International Classes:
C12Q1/68; G01N21/64; G01N33/53; G01N33/533; G02B21/00; (IPC1-7): C12Q1/68; G01N33/53; G01N33/533; G02B21/00; //G01N21/64
Domestic Patent References:
| JP11502608A | ||||
| JP8242858A | ||||
| JP2000125900A |
Foreign References:
| WO1990012115A1 | ||||
| WO2000049180A1 |
Other References:
Kinjo, M.,BioTechniques,1998年,Vol.25, No.4,p.706-715
Rigler, R.,J. Biotechnology,1995年,Vol.41,p.177-186
Rigler, R.,J. Biotechnology,1995年,Vol.41,p.177-186
Attorney, Agent or Firm:
Takehiko Suzue
Sadao Muramatsu
Tetsuya Kazama
Koji Mizuno
Sadao Muramatsu
Tetsuya Kazama
Koji Mizuno
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