METHOD FOR ISOLATING DNA FROM BIOLOGICAL MATERIAL - MAX PLANCK G ZUR FOERDERUNG WISSENSCHAFT EV

Title:

METHOD FOR ISOLATING DNA FROM BIOLOGICAL MATERIAL

Document Type and Number:

Japanese Patent JP2010279374

Kind Code:

A

Abstract:

To provide a reagent kit suitable for carrying out a method designed for stabilizing, purifying and/or isolating a nucleic acid from biological materials, especially from stool samples that can contain impurities and interfering substances.

The method comprises purifying, stabilizing and/or isolating a nucleic acid from biological materials wherein an adsorption matrix (an insoluble carbohydrate-based adsorption matrix) in order to bind impurities thereto and an extraction buffer are added to a nucleic acid-containing sample, followed by removing the nucleic acid from the adsorption matrix, and wherein the extraction buffer has: (a) a 2-8 pH range; and (b) salt concentration of at least 100 mM, and/or contains (c) a phenol-neutralizing substance.

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Inventors:

MUELLER OLIVER
SPRENGER-HAUSSELS MARKUS
BASTIAN HELGE
VOLLERT STEFANIE

Application Number:

JP2010165082A

Publication Date:

December 16, 2010

Filing Date:

July 22, 2010

Export Citation:

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Assignee:

MAX PLANCK G ZUR FOERDERUNG WISSENSCHAFT EV
QIAGEN GMBH

International Classes:

C12N15/09; C12N15/10; C12Q1/68; C12Q1/6806

Attorney, Agent or Firm:

Patent Service Corporation Patent Office Sykes

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