Title:
METHOD FOR PRODUCING THREE-DIMENSIONAL CELL STRUCTURE
Document Type and Number:
Japanese Patent JP2010068766
Kind Code:
A
Abstract:
To provide a simple method for producing a high-density three-dimensional cell structure having a uniform diameter.
The method for producing a three-dimensional cell structure includes a process (A) for concentrating and separating a cell population having a survival rate of 70% by a density gradient centrifugation method, a process (B) for seeding the cell population obtained by the process (A) on a substrate having a small region with an effective bottom area of 0.01-0.1 mm2 and a process (C) for culturing the cell seeded by the process (B).
Inventors:
ICCHODA YOKO
TAZAKI TAKESHI
TSURUTA HITOSHI
FUKUDA MOTOHIRO
TAZAKI TAKESHI
TSURUTA HITOSHI
FUKUDA MOTOHIRO
Application Number:
JP2008241029A
Publication Date:
April 02, 2010
Filing Date:
September 19, 2008
Export Citation:
Assignee:
KURARAY CO
International Classes:
C12N5/07
Domestic Patent References:
| JP2004208692A | 2004-07-29 | |||
| JP2005204523A | 2005-08-04 | |||
| JP2006512046A | 2006-04-13 | |||
| JPH04501657A | 1992-03-26 | |||
| JP2004208692A | 2004-07-29 | |||
| JP2005204523A | 2005-08-04 |
Other References:
JPN6013013748; Ｚｏｏｌ． Ｓｃｉ．， １９９１， Ｖｏｌ．８， ｐ．７１３-７１９
JPN6013013750; Ｏｒｇａｎ Ｂｉｏｌｏｇｙ， ２００１， Ｖｏｌ．８， Ｎｏ．２， ｐ．１３６
JPN6013013750; Ｏｒｇａｎ Ｂｉｏｌｏｇｙ， ２００１， Ｖｏｌ．８， Ｎｏ．２， ｐ．１３６
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