METHOD FOR QUANTIFYING NUCLEAR MIGRATION LEVEL OF NUCLEIC ACID TRANSFERRED INTO CELL

Title:

METHOD FOR QUANTIFYING NUCLEAR MIGRATION LEVEL OF NUCLEIC ACID TRANSFERRED INTO CELL

Document Type and Number:

Japanese Patent JP2006197824

Kind Code:

A

Abstract:

To provide a method for accurately quantifying the nuclear migration level of a nucleic acid transferred into a cell.

The method comprises (a) the step of fractionating a nuclear fraction from a cell transferred with a cationic conjugate comprising the objective nucleic acid and a cationic substance, (b) the step of washing the nuclear fraction fractionated in the step(a) to remove a cationic conjugate adhered to nuclear membrane and (c) the step of quantifying the objective nucleic acid contained in the nuclear fraction washed in the step(b).

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Inventors:

AKITA HIDETAKA
IWASA AKITADA
HARASHIMA HIDEYOSHI

Application Number:

JP2005010997A

Publication Date:

August 03, 2006

Filing Date:

January 18, 2005

Export Citation:

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Assignee:

UNIV HOKKAIDO

International Classes:

C12Q1/68; C12N15/09

Attorney, Agent or Firm:

Yuji Hayakawa
Suzuki Keiyasu

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